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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

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Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
11:05

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

Recent developments in peptide stereoisomer separations by capillary electromigration techniques.

Gerhard K E Scriba1

  • 1School of Pharmacy, Department of Pharmaceutical Chemistry, Friedrich Schiller University of Jena, Jena, Germany. gerhard.scriba@uni-jena.de

Electrophoresis
|June 12, 2009
PubMed
Summary
This summary is machine-generated.

Separating peptide stereoisomers is crucial for understanding their activity. Capillary electromigration techniques effectively distinguish between peptide enantiomers and diastereomers, aiding in mechanistic studies.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Separation Science

Background:

  • Peptide stereochemistry dictates biological and physicochemical properties.
  • Accurate discrimination between peptide stereoisomers is essential for research.
  • Capillary electromigration techniques are well-suited for separating hydrophilic peptide enantiomers and diastereomers.

Purpose of the Study:

  • To review recent advancements in separating peptide and peptidomimetic stereoisomers using capillary electromigration.
  • To explore mechanistic aspects of peptide enantioseparations.
  • To discuss the role of NMR and molecular modeling in understanding peptide-CD complexes.

Main Methods:

  • Capillary electromigration techniques (e.g., capillary electrophoresis).
  • NMR spectroscopy.
  • Molecular modeling.
  • Investigation of buffer additive effects on complex formation.

Main Results:

  • Peptide enantiomers exhibit unique, pH-dependent migration behaviors in cyclodextrin-mediated enantioseparations.
  • Recent developments have improved the separation of peptide stereoisomers.
  • Mechanistic insights into enantioseparations are being gained through advanced studies.

Conclusions:

  • Capillary electromigration techniques are powerful tools for peptide stereoisomer separation.
  • Understanding the mechanisms of enantioseparation is advancing through integrated analytical and computational approaches.
  • Further research into peptide-cyclodextrin interactions can enhance separation strategies.