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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

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Updated: Jun 22, 2026

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

Affinity in electrophoresis.

Niels H H Heegaard1

  • 1Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Copenhagen, Denmark. nhe@ssi.dk

Electrophoresis
|June 12, 2009
PubMed
Summary

The journal Electrophoresis advanced biomolecular affinity studies by publishing key native gel electrophoresis and capillary zone electrophoresis research. This review highlights the journal's impact on these essential separation techniques.

Area of Science:

  • Biochemistry and Biophysics
  • Analytical Chemistry
  • Molecular Biology

Background:

  • The journal Electrophoresis has been instrumental in advancing biomolecular affinity studies.
  • Native gel electrophoresis and capillary zone electrophoresis (CE) are central techniques for studying biomolecule interactions.
  • The author's research heavily relies on methods frequently featured in Electrophoresis.

Observation:

  • Electrophoresis has published numerous articles on native gel electrophoresis and capillary zone electrophoresis.
  • These publications have significantly shaped the development and application of these techniques.
  • The journal has served as a primary platform for disseminating knowledge in affinity electrophoresis and affinity CE.

Findings:

  • The journal Electrophoresis has played a crucial role in the evolution of native gel electrophoresis and capillary zone electrophoresis.

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An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

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Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

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Last Updated: Jun 22, 2026

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

  • Key papers in Electrophoresis have guided the author's methodological choices for biomolecular affinity studies.
  • The journal's content provides a historical perspective on the development of these separation sciences.

    • Implications:

    • Understanding the historical contributions of Electrophoresis enhances the interpretation of current research in biomolecular interactions.
    • The journal's legacy supports the continued development and application of electrophoresis techniques in life sciences.
    • This review underscores the importance of specialized journals in fostering scientific progress and technique dissemination.