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Liposomes in capillary electromigration techniques.

Susanne K Wiedmer¹, Ruth Shimmo

¹Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Finland. susanne.wiedmer@helsinki.fi

Electrophoresis

|June 12, 2009

Summary

This review covers phospholipid vesicles (liposomes) in electrophoresis and chromatography. It details liposome applications for separating neutral and charged compounds and their use in studying lipid-analyte interactions.

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Area of Science:

Analytical Chemistry
Separation Science

Background:

Phospholipid vesicles, or liposomes, are increasingly utilized in various separation techniques.
Capillary electrophoresis (CE) and capillary electrochromatography (CEC) offer unique platforms for liposome applications.

Purpose of the Study:

To review the applications of liposomes in EKC and CEC.
To discuss the use of CE for analyzing liposomes and lipid-analyte aggregates.

Main Methods:

Review of literature on liposome dispersions in EKC for separating neutral and charged compounds.
Analysis of CEC methods involving immobilized liposomes for analyte interaction control and chromatographic phase development.
Overview of CE techniques for studying liposomes and lipid-analyte complexes.

Main Results:

Common liposome dispersions effective for EKC separations are identified.
Two distinct strategies for immobilizing liposomes in CEC are presented: preventing analyte-wall interactions and creating chromatographic phases.
CE proves valuable for investigating liposomes and lipid-analyte aggregates.

Conclusions:

Liposomes are versatile tools in EKC and CEC for compound separation.
Immobilized liposomes offer tunable selectivity in CEC.
CE is a suitable method for characterizing liposomes and related aggregates.