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Updated: Jun 22, 2026
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
[Genoserotyping of Listeria monocytogenes in multiplex PCR]
Grzegorz Madajczak1, Danuta Majczyna
1Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego--Państwowego Zakładu Higieny w Warszawie.
Abstract:
Listeria monocytogenes infection affects people throughout the all world, most often causing gastrointestinal disturbances, less inflammation of the central nervous system (meningitis, meningoencephalitis, absceses), sepsis, endocarditis and dispersed focal inflammation. Listeria monocytogenes infection in pregnant woman can lead also to premature birth or abortion. Listeriosis is a serious problem because of the high mortality in case of generalized infection, which can reach up to 20%. Listeria strains could be identified by determination of biochemical features--characteristic for each Listeria species. Biochemically identified strains could be serotyped using Seeliger method. Many authors developed a molecular biology-based methods of Listeria serotype determination The Doumith's method divide all Listeria monocytogenes serotypes for five genoserogroups. The purpose of this study was to determine the genoserogroups of Listeria monocytogenes strains and compare those results with results obtained by classical serotyping by Seeliger method. To this work 90 Listeria strains were used: 75 Listeria monocytogenes strains from clinical, food and environmental samples and 15 reference strains from Pasteur Institute collection and National Institute of Public Health--National Institute of Hygiene collection. All strains were serotyped using liquid stable antisera for determination of O and H Listeria monocytogenes antigens (Mast Diagnostic, UK). Multiplex PCR for Listeria monocytogenes serovars differentiation were performed according to Doumith et al procedure. The genoserogroup obtained from multiplex PCR of 10 Listeria monocytogenes reference strains were compatible with real serotype. In case of non-Listeria monocytogenes reference strains in multiplex-PCR, they were identified as non-monocytogenes. In group of 75 non-reference strains isolated from human, food and environmental sources, 34 belonged to genoserogroup I.1, two strains to genoserogroup I.2, 11 strains to genoserogroup II.1, 21 strains belonged to genoserogroup II.2 and 9 strains to genoserogroup III.
Insights
Listeria monocytogenes infections pose a global health risk. This study compared molecular genoserotyping with traditional serotyping for Listeria strains, finding genoserotyping effective for identifying Listeria monocytogenes.
Area of Science:
- Microbiology
- Molecular Biology
- Infectious Diseases
Context:
- Listeria monocytogenes causes severe infections globally, with high mortality rates.
- Accurate identification and serotyping of Listeria strains are crucial for public health.
- Classical biochemical and serological methods have limitations in differentiating Listeria species and serotypes.
Purpose:
- To determine the genoserogroups of Listeria monocytogenes strains using multiplex PCR.
- To compare the results of molecular genoserotyping with classical serotyping methods (Seeliger method).
- To evaluate the accuracy of Doumith's method for Listeria monocytogenes serotype determination.
Summary:
- Ninety Listeria strains, including clinical, food, and environmental isolates, were analyzed.
- Multiplex PCR based on Doumith's method was used for genoserotyping.
- Results showed compatibility between genoserogroups and serotypes for reference strains, with specific genoserogroup distributions observed in non-reference isolates.
Impact:
- This study validates a molecular method for rapid and accurate Listeria monocytogenes serotyping.
- The findings contribute to improved diagnostics and surveillance of listeriosis.
- Effective strain differentiation aids in understanding transmission routes and controlling outbreaks.
