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Related Concept Videos

Rab Proteins01:14

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

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Related Experiment Video

Updated: Jun 22, 2026

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
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Characterization of rabbit CD5 isoforms.

Richard Pospisil1, Juraj Kabat, Rose G Mage

  • 1Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1892, USA.

Molecular Immunology
|June 13, 2009
PubMed
Summary
This summary is machine-generated.

The monoclonal antibody KEN-5 recognizes a CD5-like molecule on rabbit T cells and a small subset of B cells, with binding influenced by glycosylation. This suggests differential CD5 glycosylation impacts T and B cell populations.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Previous studies indicated rabbit CD5 antibodies bind most B cells.
  • The monoclonal antibody (mAb) KEN-5 was reported to recognize rabbit CD5.

Purpose of the Study:

  • To investigate the binding characteristics of mAb KEN-5 to rabbit CD5.
  • To explore the role of glycosylation in differential CD5 recognition on T and B cells.

Main Methods:

  • Enzyme-linked Immunosorbent Assay (ELISA) for binding analysis.
  • Immunoprecipitation to isolate CD5 molecules.
  • Flow cytometry and immunohistochemistry for cellular localization.
  • Deglycosylation assays to assess glycan influence.

Main Results:

  • KEN-5 binds recombinant rabbit CD5, with partial inhibition by anti-CD5 polyclonal antibody.
  • KEN-5 primarily recognizes T cells and a minor population (3-6%) of IgM(+) B cells.
  • Deglycosylation reduces KEN-5 binding, suggesting glycan structures influence the epitope.

Conclusions:

  • KEN-5 recognizes a CD5-like molecule with differential binding to T and B cells, likely due to distinct glycosylation patterns.
  • Differential CD5 glycosylation may influence T and B cell subset development, selection, or survival.