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Standard calibration proteins for Western blotting obtained by genetically prepared protein A conjugates
C Lindbladh1, K Mosbach, L Bülow
1Department of Pure and Applied Biochemistry, University of Lund, Sweden.
Analytical Biochemistry
|August 15, 1991
Abstract:
Genetically prepared protein A fusion proteins, having retained antibody binding capacity, were used to design different well-defined standard molecular weight marker proteins for Western blotting. The blotted marker proteins are developed at the same time and with the same reagents as the protein sample of interest.