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Updated: Jun 22, 2026

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
Published on: February 27, 2012
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection
Shane J F Cronin1, Nadine T Nehme, Stefanie Limmer
1IMBA, Institute of Molecular Biotechnology of the Austrian Academy of Sciences, A-1030 Vienna, Austria.
Abstract:
Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. Therefore, we revealed multiple genes involved in antibacterial defense and the regulation of innate immunity.
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