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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

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Related Experiment Video

Updated: Jun 22, 2026

Real-time Live Imaging of T-cell Signaling Complex Formation
10:31

Real-time Live Imaging of T-cell Signaling Complex Formation

Published on: June 23, 2013

Visualizing intermolecular interactions in T cells.

Nicholas R J Gascoigne1, Jeanette Ampudia, Jean-Pierre Clamme

  • 1Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. Gascoigne@scripps.edu

Current Topics in Microbiology and Immunology
|June 13, 2009
PubMed
Summary
This summary is machine-generated.

Förster Resonance Energy Transfer (FRET) microscopy visualizes protein interactions in living cells. This study used FRET to analyze T cell receptor and coreceptor CD4/CD8 interactions at the immunological synapse.

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A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
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Published on: March 22, 2012

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A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

Area of Science:

  • Cellular biology
  • Immunology
  • Biophysics

Background:

  • Fluorescent proteins enable Förster Resonance Energy Transfer (FRET) microscopy for studying molecular interactions.
  • FRET microscopy offers dynamic, subcellular insights into protein interactions within living cells.

Purpose of the Study:

  • To investigate intermolecular interactions between the T cell antigen receptor (TCR) and coreceptors CD4 and CD8.
  • To analyze the recruitment and interaction dynamics of CD4 and CD8 within the immunological synapse.

Main Methods:

  • Utilized FRET microscopy to visualize and quantify protein interactions in real-time.
  • Applied FRET microscopy to living cells to observe subcellular localization and dynamics.

Main Results:

  • Demonstrated the dynamic recruitment of CD4 and CD8 coreceptors to the immunological synapse.
  • Showcased distinct interaction patterns between TCR, CD4, and CD8 upon T cell stimulation with varying ligands.

Conclusions:

  • FRET microscopy is a powerful tool for dissecting dynamic protein interactions in cellular immunology.
  • The study provides insights into the spatial and temporal regulation of TCR and coreceptor engagement during T cell activation.