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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Genetic diversity and population structure of Celosia argentea and related species revealed by SRAP
Na Feng1, Qie Xue, Qinghua Guo
1Department of Pharmacognosy, College of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, China.
Abstract:
Genetic diversity of 16 populations of Celosia argentea L. and 6 populations of Celosia cristata L. in China was investigated using sequence-related amplified polymorphism (SRAP). Ten SRAP primer combinations generated 507 scorable amplification bands ranging from 50 to 2000 bp, among which 274 were polymorphic, with an average of 54 polymorphic bands per primer combination. The unweighted pair group method of arithmetic averages (UPGMA) cluster analysis enabled construction of a phylogenetic tree for estimating genetic distance among populations, which agreed well with the geographic origin information. Twenty-two populations were distinctly separated into two major genetic groups. One typical representative fragment, M1E6 in C. argentea, provided an alternative approach to distinguish C. argentea from C. cristata. Also, great genetic diversity found in C. argentea populations by significant geographic difference was confirmed by a high level of population genetics parameters. The information may be beneficial to future breeding selection and conservation management for populations of C. argentea.
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