Related Experiment Video
Updated: Jun 22, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
[Evaluation of HbA1c using different methods in haemoglobin variant, Hb J-Bangkok]
Wakana Sawaragi1, Hitoshi Shibuya, Keiko Yasuda
1Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo 060-8648, Japan. xswakana@med.hokudai.ac.jp
Abstract:
A 31-year-old Japanese man with haemoglobin variant, Hb J-Bangkok [beta56 (D7) Gly-->Asp], was found by discrepant values between HbA1c and glycated-albumin. We measured HbA1c using three different methods, HPLC, enzyme assay and turbidimetric immunoassay. HbA1c value measured by HPLC was much lower than those by others. Furthermore, we estimated calculated glyco-haemoglobin value measured by high-resolution HPLC, revealing that HbA1c values measured by enzyme assay and turbidimetric immunoassay were comparable with calculated value. When measuring HbA1c value in haemoglobin variant, Hb J Bangkok, enzyme assay and turbidimetric immunoassay are useful methods.
Related Concept Videos
Type II Diabetes Mellitus III: Clinical Manifestations and Diagnosis
Hyperglycemia
Blood Studies I: ABG and VBG
Arterial Blood Gas (ABG)
Arterial Blood Gas (ABG) studies are crucial for assessing the lungs' ability to supply oxygen and remove carbon dioxide, reflecting the patient's ventilation status. They also help understand the kidneys' capacity to reabsorb or...
