Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 22, 2026

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
08:24

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

Published on: April 18, 2017

The zebrafish as a model system for forebrain GnRH neuronal development.

Eytan Abraham1, Ori Palevitch, Yoav Gothilf

  • 1Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD, USA.

General and Comparative Endocrinology
|June 16, 2009
PubMed
Summary

The novel transgenic zebrafish model allows in vivo study of gonadotropin-releasing hormone (GnRH) neurons, crucial for reproduction and neuronal migration. This tool aids research into GnRH functions and system development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-nuclei RNA sequencing reveals heterogeneity within developing GnRH3 neurons in zebrafish.

Journal of neuroendocrinology·2026
Same author

Rethinking CAR-T manufacturing paradigms: terminology, operational considerations, and economic trade-offs.

Cytotherapy·2026
Same author

INTS1 is required for maintaining accurate transcriptional integrity and behavior in zebrafish.

NAR genomics and bioinformatics·2025
Same author

A simple and rapid shaking-based assay to genotype live, early developmental stage zebrafish embryos.

Laboratory animals·2025
Same author

The snakehead retrovirus promoter functions independently of the 3'ORF protein and its products are maternally inherited in transgenic zebrafish.

PLoS pathogens·2025
Same author

Zebrafish Dark-Dependent Behavior Requires Phototransduction by the Pineal Gland.

Journal of pineal research·2024

Area of Science:

  • Neuroscience
  • Endocrinology
  • Developmental Biology

Background:

  • The forebrain gonadotropin-releasing hormone (GnRH) system is vital for vertebrate reproduction and serves as a model for neuronal migration.
  • Multiple GnRH forms and widespread projections suggest diverse functions beyond reproduction.
  • Studying the GnRH system is challenging due to limitations in existing model systems and methodologies.

Purpose of the Study:

  • To introduce and discuss the utility of the transgenic (Tg) GnRH3:EGFP zebrafish line for in vivo investigation of the GnRH system.
  • To highlight the advantages of using zebrafish as a model for studying forebrain GnRH neurons.
  • To explore the potential of this model for understanding GnRH3 system development and regulation.

Main Methods:

  • Utilizing the Tg(GnRH3:EGFP) zebrafish line, where GnRH3 neurons express enhanced green fluorescent protein (EGFP).

More Related Videos

Forebrain Electrophysiological Recording in Larval Zebrafish
06:00

Forebrain Electrophysiological Recording in Larval Zebrafish

Published on: January 24, 2013

Related Experiment Videos

Last Updated: Jun 22, 2026

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
08:24

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

Published on: April 18, 2017

Forebrain Electrophysiological Recording in Larval Zebrafish
06:00

Forebrain Electrophysiological Recording in Larval Zebrafish

Published on: January 24, 2013

  • In vivo imaging and analysis of GnRH3 neurons in developing larvae and sexually mature zebrafish.
  • Leveraging the genetic and molecular tools available in the zebrafish model.

    • Main Results:

    • The Tg(GnRH3:EGFP) zebrafish line enables real-time, whole-animal visualization of GnRH3 neurons.
    • This model facilitates the study of GnRH neuron development, migration, and function throughout the animal's life.
    • The zebrafish system provides a powerful platform for investigating the regulation of the GnRH system.

    Conclusions:

    • The Tg(GnRH3:EGFP) zebrafish line is an effective model for studying forebrain GnRH neurons in vivo.
    • This model expands research capabilities for understanding GnRH system development and broader functions.
    • Zebrafish offer a valuable vertebrate model for advancing GnRH neurobiology research.