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Updated: Jun 22, 2026

Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
Published on: March 1, 2014
Continuous uninterrupted single medium culture without medium renewal versus sequential media culture: a sibling
Michael L Reed1, Amanda Hamic, Douglas J Thompson
1Center for Reproductive Medicine of New Mexico, Albuquerque, New Mexico 87106, USA. mleroyreed@yahoo.com
Abstract:
Eight hundred ninety-three sibling embryos from 80 IVF cycles were cultured side by side in either: 1) a single medium continuously, without medium renewal on day 3; or 2) sequential media. There were no significant differences between the two culture media systems regarding embryo quality or the proportion of embryos selected for transfer on day 3 from either media; however, for day 5 embryo transfer, a greater number of blastocysts were available, and were selected for transfer, from the continuous single medium culture compared with sequential media culture.
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