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Updated: Feb 10, 2026

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
Published on: February 25, 2017
Quantitative analysis of gene expression in a single cell by qPCR
Kiyomi Taniguchi1, Tomoharu Kajiyama, Hideki Kambara
1Hitachi Central Research Laboratory, Tokyo, Japan.
Abstract:
We developed a quantitative PCR method featuring a reusable single-cell cDNA library immobilized on beads for measuring the expression of multiple genes in a single cell. We used this method to analyze multiple cDNA targets (from several copies to several hundred thousand copies) with an experimental error of 15.9% or less. This method is sufficiently accurate to investigate the heterogeneity of single cells.
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