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A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
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Lamin B content in chromatin fractions after purification of nuclear matrix from cells of different types

M A Lapshina1, I I Parkhomenko, R I Papina

  • 1Laboratory of Molecular Biology, Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Russia. lapshina@icp.ac.ru

Bulletin of Experimental Biology and Medicine
|June 16, 2009
PubMed

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The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
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