Quantification of thyrotropin-releasing hormone by liquid chromatography-electrospray mass spectrometry
Angela Chambery1, Valeria Severino, Antimo Di Maro
1Dipartimento di Scienze della Vita, Seconda Università di Napoli, Via Vivaldi 43, 81100, Caserta, Italy. angela.chambery@unina2.it
Abstract:
Thyrotropin-releasing hormone (TRH) is involved in a wide range of biological responses. It has a central role in the endocrine system and regulates several neurobiological activities. In the present study, a rapid, sensitive and selective liquid chromatography-mass spectrometry method for the identification and quantification of TRH has been developed. The methodology takes advantage of the specificity of the selected-ion monitoring acquisition mode with a limit of detection of 1 fmol. Furthermore, the MS/MS fragmentation pattern of TRH has been investigated to develop a selected reaction monitoring (SRM) method that allows the detection of a specific b2 product ion at m/z 249.1, corresponding to the N-terminus dipeptide pyroglutamic acid-histidine. The method has been tested on rat hypothalami to evaluate its suitability for the detection within very complex biological samples.
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