Approaching clinical proteomics: current state and future fields of application in fluid proteomics

Area of Science:

• Clinical proteomics
• Biomarker discovery
• Fluid proteomics

Background:

• Clinical proteomics aims to identify novel disease biomarkers in biological samples.
• Technological advancements in protein arrays and spectrometry enable clinical diagnostic potential.
• The dynamic range and complexity of body fluid proteomes pose significant quantification challenges.

Purpose of the Study:

• To review recent technological advances and applications in clinical proteomics, focusing on fluid proteomics.
• To highlight the importance of preanalytical standardization and quality control for clinical implementation.
• To discuss the integration of proteomic data into clinical decision-making.

Main Methods:

• Review of recent technological advancements in proteomics.
• Analysis of preanalytical factors affecting fluid proteomics.
• Discussion of analytical standardization and quality control measures.

Main Results:

• New protein array and spectrometry technologies show promise for clinical diagnostics.
• Preanalytical variables significantly influence proteomics study results, especially in fluid samples.
• Standardization of preanalytical phases is essential for reliable proteomic analysis.

Conclusions:

• Technological progress supports clinical proteomics, particularly fluid proteomics.
• Improved standardization of preanalytical steps, sample handling, and data analysis is critical.
• Further clinical studies are needed to validate biomarker candidates and their impact on clinical decision-making.