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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

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Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates
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Induced pluripotent stem cells at nanoscale.

Dagmar Zeuschner1, Karina Mildner, Holm Zaehres

  • 1Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine , Münster, Germany.

Stem Cells and Development
|June 17, 2009
PubMed
Summary

Induced pluripotent stem (iPS) cells reprogrammed using Oct4, Sox2, c-Myc, and Klf4 closely resemble embryonic stem cells. Electron microscopy confirmed their ultrastructural similarity, supporting their comparable pluripotency.

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Published on: January 8, 2017

Area of Science:

  • Stem cell biology
  • Cellular reprogramming
  • Molecular biology

Background:

  • Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells.
  • The transcription factor quartet Oct4, Sox2, c-Myc, and Klf4 drives this reprogramming.
  • Pluripotency of iPS cells is a key area of research.

Purpose of the Study:

  • To compare the ultrastructural characteristics of iPS cells and embryonic stem cells (ESCs).
  • To provide further evidence for the similarity between iPS cells and ESCs.

Main Methods:

  • Generation of iPS cells from mouse embryonic fibroblasts using Oct4, Sox2, c-Myc, and Klf4.
  • Comparison of iPS cells and ESCs using electron microscopy.

Main Results:

  • Ultrastructural analysis revealed striking similarities between iPS cells and ESCs.
  • No significant ultrastructural differences were observed between the two cell types.

Conclusions:

  • iPS cells generated with the four factors are ultrastructurally indistinguishable from ESCs.
  • This finding supports the functional similarity and pluripotency of these stem cell populations.