Related Experiment Video
Updated: Aug 12, 2026
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Murine Dermal Fibroblast Isolation by FACS
Published on: January 7, 2016
Antiseptic effectiveness with fibroblast preservation
P J McKenna1, G S Lehr, P Leist
1Department of Surgery, Good Samaritan Hospital, Cincinnati, OH.
Annals of Plastic Surgery
|September 1, 1991
Summary
0.005% sodium hypochlorite effectively treats wounds and skin ulcers by inhibiting bacteria without harming fibroblast function, crucial for healing. This topical antiseptic offers a safe and effective wound care solution.
Area of Science:
- Wound healing research
- Antiseptic efficacy
- Microbiology
Background:
- Topical antiseptics can impair fibroblast and leukocyte function, hindering wound healing.
- Fibroblast function is concentration-dependent when exposed to antiseptics.
Purpose of the Study:
- To evaluate the efficacy of low-concentration antiseptics against clinical bacterial isolates.
- To determine if antiseptics preserving fibroblast function can be used for wound care.
Main Methods:
- Tested 0.005% sodium hypochlorite, 0.001% povidone-iodine, 0.0025% acetic acid, and 0.003% hydrogen peroxide.
- Assessed antiseptic effectiveness against Staphylococcus aureus, Escherichia coli, Group D enterococci, Pseudomonas aeruginosa, and Bacteroides fragilis.
- Measured bacterial inhibition through standard microbiological plating techniques.
Main Results:
- 0.005% sodium hypochlorite inhibited all tested bacteria (p < 0.001).
- Povidone-iodine reduced S. aureus colonies; acetic acid inhibited P. aeruginosa.
- Concentrations tested preserved fibroblast function.
Conclusions:
- 0.005% sodium hypochlorite demonstrates potent antibacterial activity against common wound pathogens.
- This concentration of sodium hypochlorite can serve as a debriding and topical antibacterial agent for wounds and skin ulcers.
- It supports normal wound repair by not inhibiting essential fibroblast activity.
