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Improving the enrichment procedure for Enterobacteriaceae detection.

Christine Weber1, Roger Stephan, Patrick Druggan

  • 1Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 272, Zurich CH-8057, Switzerland.

Food Microbiology
|June 17, 2009
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Summary

This study enhances the Enterobacteriaceae detection method by modifying the pre-enrichment broth. Supplementation improved recovery of target bacteria, offering a more effective approach for food safety testing.

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Area of Science:

  • Microbiology
  • Food Science
  • Analytical Chemistry

Background:

  • The ISO standard method (21528-1:2004) for Enterobacteriaceae detection uses EE broth, which inhibits some species like Cronobacter spp.
  • A modified procedure omitting EE broth faces challenges with Gram-positive flora competition, hindering low-level target organism recovery.

Purpose of the Study:

  • To investigate cost-effective modifications to improve the ISO 21528-1:2004 method for Enterobacteriaceae detection.
  • To address limitations of existing methods, particularly the inhibitory nature of EE broth and competition from background flora.

Main Methods:

  • Experiments utilized stressed Enterobacteriaceae strains and background competitors from powdered milk.
  • Investigated the interaction between Enterobacteriaceae and competitors.
  • Evaluated additives to enhance target strain growth over non-target strains in pre-enrichment broth.

Main Results:

  • Supplementation of Buffered Peptone Water (BPW) with specific additives (8-hydroxyquinoline, ammonium iron(III) citrate, sodium deoxycholate, sodium pyruvate) created a modified broth (BPW-S).
  • BPW-S significantly improved the recovery of Enterobacteriaceae from both artificially and naturally contaminated samples.
  • The enhanced pre-enrichment broth demonstrated effectiveness even with stressed target strains and competitive flora.

Conclusions:

  • The developed BPW-S formulation offers a cost-effective improvement to the standard Enterobacteriaceae detection method.
  • This enhanced pre-enrichment strategy is beneficial for detecting Enterobacteriaceae, including specific foodborne pathogens like Salmonella spp. and Cronobacter spp.
  • The findings provide a valuable alternative for improving microbial detection in food matrices.