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Updated: Jun 22, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
Fluorescence quenching properties of multiple pyrene-modified RNAs
Kenji Maie1, Mitsunobu Nakamura, Tadao Takada
1Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
Abstract:
RNA molecules with multiple pyrenylmethyl substituents on the 2'-O-sugar residues can form duplexes with complementary RNA sequences without losing thermal stability. In the RNA duplexes, covalently incorporated pyrenes can assemble in a helical manner along the minor grooves of the duplex. These helically assembled pyrene arrays exhibit intense excimer emissions that are efficiently quenched with methyl viologen.
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