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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Current-generation high-throughput sequencing: deepening insights into mammalian transcriptomes.

Benjamin J Blencowe1, Sidrah Ahmad, Leo J Lee

  • 1Banting and Best Department of Medical Research, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada. b.blencowe@utoronto.ca

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High-throughput sequencing (HTS) reveals new RNA complexity and processing insights. This powerful technology enhances transcriptome profiling accuracy and coverage, advancing our understanding of cellular networks.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • High-throughput sequencing (HTS) is a transformative technology for biological research.
  • Transcriptome characterization is crucial for understanding gene expression and cellular function.

Purpose of the Study:

  • To review the initial applications and findings of HTS in transcriptome characterization.
  • To discuss the strengths and limitations of HTS compared to microarray profiling.
  • To explore the future impact of HTS on understanding RNA-level cellular networks.

Main Methods:

  • Application of high-throughput sequencing (HTS) technologies.
  • Profiling of mammalian transcriptomes.
  • Comparative analysis with microarray profiling.

Main Results:

  • Detection of significant new transcript complexity.
  • Elucidation of RNA-binding protein binding maps and regulatory roles.
  • New insights into pre-mRNA processing steps.
  • Demonstration of HTS's high quantitative accuracy and profiling coverage.

Conclusions:

  • HTS offers unprecedented power for transcriptome analysis.
  • Future HTS advancements will deepen our understanding of integrated cellular networks at the RNA level.
  • HTS provides a more comprehensive view of the transcriptome than previous methods.