Related Experiment Video
Updated: Jun 22, 2026
Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
Published on: April 21, 2022
Osteopontin modulates malignant pleural mesothelioma cell functions in vitro
Rina Ohashi1, Ken Tajima, Fumiyuki Takahashi
1Department of Respiratory Medicine, Juntendo University, School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan. rinaohas@juntendo.ac.jp
Background:
Although serum osteopontin (OPN) concentration is elevated in patients with malignant pleural mesothelioma (MPM), the role of OPN in the pathogenesis and development of MPM remains unknown.
Materials And Methods:
To determine the roles of OPN in MPM, immunohistochemical staining was performed to investigate the concentration of OPN in the pleural tumor of patients with mesothelioma; cell adhesion, proliferation and migration assays of H28 cells, an MPM cell line, were also carried out in vitro.
Results:
H28 cells cultured on OPN-coated plates revealed enhanced adhesion, proliferation, migration, cell survival and phosphorylated focal adhesion kinase activities. As expected, these enhancements were markedly suppressed with the addition of anti-alphavbeta3 antibody or arginine-glycine-aspartic acid serine (RGDS) peptide to the medium.
Conclusion:
OPN is speculated to play an important role in the enhancement of adhesion, proliferation and migration activities of H28 cells, presumably by interacting with the alphavbeta3 integrin.
