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In vitro enzymatic synthesis of new penicillins containing keto acids as side chains
M A Ferrero1, A Reglero, H Martínez-Blanco
1Departamento Interfacultativo de Bioquímica y Biología Molecular, Facultades de Veterinaria y Biología, Universidad de León, Spain.
Antimicrobial Agents and Chemotherapy
|September 1, 1991
Abstract:
Seven different penicillins containing alpha-ketobutyric, beta-ketobutyric, gamma-ketovaleric, alpha-ketohexanoic, delta-ketohexanoic, epsilon-ketoheptanoic, and alpha-ketooctanoic acids as side chains have been synthesized in vitro by incubating the enzymes phenylacetyl coenzyme A (CoA) ligase from Pseudomonas putida and acyl-CoA:6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum with CoA, ATP, Mg(2+), dithiothreitol, 6-aminopenicillanic acid, and the corresponding side chain precursor.