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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
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Published on: March 15, 2021

X-ray ultramicroscopy using integrated sample cells.

Dachao Gao, Stephen W Wilkins, David J Parry

    Optics Express
    |June 17, 2009
    PubMed
    Summary
    This summary is machine-generated.

    A new X-ray ultramicroscope (XuM) design enhances X-ray microscopy. Integrated sample cells improve stability and enable high-resolution imaging of diverse samples, from dry to liquid.

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    Area of Science:

    • Physics
    • Materials Science
    • Microscopy

    Background:

    • X-ray ultramicroscopy (XuM) offers a novel approach to X-ray projection microscopy.
    • Existing XuM techniques can be limited by instrumentation complexity and sample handling.

    Purpose of the Study:

    • To introduce and evaluate right-angle-type integrated sample cells for the X-ray ultramicroscope (XuM).
    • To demonstrate the expanded capabilities and improved performance of the XuM with the new sample cell design.

    Main Methods:

    • Development of right-angle-type integrated sample cells combining target, spacer, sample chamber, and exit window.
    • Utilizing a scanning electron microscope as the host for the X-ray ultramicroscope.
    • Acquiring XuM imaging data from various sample types (dry, wet, liquid).

    Main Results:

    • The integrated sample cells simplify XuM instrumentation and enhance mechanical stability.
    • XuM imaging successfully characterized very small features down to approximately 100nm.
    • The technique proved effective for imaging dry, wet, and liquid samples.

    Conclusions:

    • Right-angle integrated sample cells significantly advance X-ray ultramicroscope capabilities.
    • The improved XuM system enables high-resolution characterization of diverse sample states.
    • This development opens new avenues for nanoscale imaging in various scientific fields.