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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 22, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Very high speed cw digital holographic interferometry.

Carlos Pérez-López, Manuel H De la Torre-Ibarra, Fernando Mendoza Santoyo

    Optics Express
    |June 17, 2009
    PubMed
    Summary

    This study introduces high-speed digital holographic interferometry for capturing out-of-plane displacement. The technique uses a 4000 fps camera to analyze dynamic movements with microsecond precision.

    Area of Science:

    • Optics and Photonics
    • Experimental Mechanics
    • Fluid Dynamics

    Background:

    • Digital holographic interferometry (DHI) is a powerful non-contact technique for measuring deformations.
    • Traditional DHI systems often have limitations in temporal resolution, restricting the analysis of fast dynamic events.
    • High-speed imaging is crucial for capturing transient phenomena in various scientific and engineering fields.

    Purpose of the Study:

    • To demonstrate the novel application of a very high-speed camera in digital holographic interferometry.
    • To achieve high temporal resolution for out-of-plane displacement measurements.
    • To validate the technique's feasibility for analyzing dynamic object movements.

    Main Methods:

    • Utilized a very high-speed camera (4000 frames per second) for image plane hologram acquisition.

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  • Employed a continuous wave (cw) laser for illuminating a spherical latex balloon.
  • Performed individual Fourier processing of sequential holograms and phase map generation via subtraction.

    • Main Results:

    • Successfully acquired holograms at 4000 fps with 250-microsecond time intervals.
    • Generated phase maps illustrating out-of-plane displacement, visualized as a movie.
    • Demonstrated the technique's capability under both unconstrained (air currents) and controlled conditions.

    Conclusions:

    • The integration of high-speed cameras significantly advances digital holographic interferometry's capabilities.
    • This method enables the study of dynamic out-of-plane displacements with unprecedented temporal resolution.
    • The technique shows promise for applications requiring the analysis of rapid object motion.