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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 22, 2026

In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation
09:39

In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation

Published on: May 27, 2013

Supercontinuum generation in an imaging fiber taper.

Kebin Shi, Fiorenzo G Omenetto, Zhiwen Liu

    Optics Express
    |June 17, 2009
    PubMed
    Summary

    Researchers generated supercontinuum spectra using individual fibers within a Schott imaging fiber taper. This novel approach enables controllable generation of supercontinuum arrays, expanding possibilities beyond single fiber methods.

    Area of Science:

    • Optics and Photonics
    • Materials Science

    Background:

    • Supercontinuum generation is crucial for various applications, typically relying on specialized fibers like photonic crystal fibers (PCFs).
    • Conventional methods often involve single PCFs or individual tapered fibers, limiting scalability and independent control.

    Purpose of the Study:

    • To investigate supercontinuum generation within individual fibers of a commercial Schott imaging fiber taper.
    • To explore the potential of using an array of fibers for novel supercontinuum generation techniques.

    Main Methods:

    • Utilized a commercial Schott imaging fiber taper.
    • Induced supercontinuum generation in individual fibers within the taper.
    • Characterized the generated supercontinuum spectrum.

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    Microwave Photonics Systems Based on Whispering-gallery-mode Resonators
    12:18

    Microwave Photonics Systems Based on Whispering-gallery-mode Resonators

    Published on: August 5, 2013

    Related Experiment Videos

    Last Updated: Jun 22, 2026

    In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation
    09:39

    In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation

    Published on: May 27, 2013

    Microwave Photonics Systems Based on Whispering-gallery-mode Resonators
    12:18

    Microwave Photonics Systems Based on Whispering-gallery-mode Resonators

    Published on: August 5, 2013

    Main Results:

    • Achieved supercontinuum generation covering a broad wavelength range from approximately 500 nm to 1 µm.
    • Demonstrated that each fiber within the imaging taper can independently generate a supercontinuum.
    • Confirmed the feasibility of creating arrays of supercontinuum spectra.

    Conclusions:

    • Supercontinuum generation is feasible in individual fibers of an imaging fiber taper.
    • This approach offers a new pathway for generating controllable supercontinuum arrays.
    • The multi-fiber nature of imaging tapers opens possibilities for advanced optical applications.