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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to the...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single stretching vibration...

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Related Experiment Video

Updated: Jun 22, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Molecular identification by generating coherence between molecular normal modes using stimulated Raman scattering.

Daniel L Marks1, Joseph B Geddes Iii, Stephen A Boppart

  • 1Beckman Institute for Advanced Science and Technology, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Optics Letters
|June 17, 2009
PubMed
Summary

We developed a method using stimulated Raman scattering to drive molecular vibrations into coherence. This technique enhances signals from target molecules, enabling better distinction from other substances in coherent Raman microspectroscopy.

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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
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Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
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Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

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Last Updated: Jun 22, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Area of Science:

  • Molecular spectroscopy
  • Quantum optics
  • Chemical analysis

Background:

  • Coherent control of molecular vibrations is crucial for advanced spectroscopy.
  • Stimulated Raman scattering (SRS) offers a non-destructive way to probe molecular vibrations.
  • Distinguishing target molecules from background noise is a key challenge in chemical analysis.

Purpose of the Study:

  • To introduce a novel method for inducing coherent vibrations in target molecules.
  • To enhance the anti-Stokes signal for improved molecular detection.
  • To develop a technique for differentiating target molecules from confounders using coherent Raman scattering.

Main Methods:

  • Utilizing tailored laser pulses to drive normal modes of vibration into coherence via stimulated Raman scattering.
  • Analyzing the resulting anti-Stokes signal intensity.
  • Comparing signal generation from target molecules versus other molecules under the same illumination.

Main Results:

  • Coherent excitation of multiple vibrational modes significantly amplifies the anti-Stokes signal compared to single-mode excitation.
  • The proposed method selectively enhances signals from target molecules, while leaving other molecules largely unaffected.
  • Demonstrated the ability to distinguish target molecules from confounders based on their distinct Raman scattering responses.

Conclusions:

  • The developed method effectively enhances molecular signals through coherent vibrational excitation.
  • This technique provides a powerful tool for selective molecular detection and identification.
  • Coherent Raman microspectroscopy can be significantly advanced for applications requiring high sensitivity and specificity.