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Updated: Jun 22, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
Published on: April 28, 2022
Coherent anti-Stokes Raman scattering microscopy using tightly focused radially polarized light
Fake Lu1, Wei Zheng, Zhiwei Huang
1Optical Bioimaging Laboratory, Department of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore 117576.
Abstract:
We report a radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy for facilitating longitudinally oriented molecules detection. We observe that under tight focusing of radially polarized pump and Stokes light fields with a high-NA objective, RP-CARS radiation from molecules oriented along the longitudinal direction is approximately threefold stronger than that using linearly polarized CARS (LP-CARS) technique. The lateral resolution of RP-CARS imaging can be improved by about 10% compared to the LP-CARS imaging. We demonstrate this RP-CARS technique by imaging the sectioned cottonwood leaf vascular bundles.
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