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Updated: Jun 22, 2026

Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
Published on: September 6, 2024
A comprehensive model of simultaneous denitrification and methanogenic fermentation processes
A Evren Tugtas1, U Tezel, Spyros G Pavlostathis
1Department of Environmental Engineering, Bahcesehir University, Istanbul, Turkey.
Abstract:
The denitrification process was incorporated into the IWA Anaerobic Digestion Model No. 1 (ADM1) in order to account for the effect of denitrification on the methanogenic fermentation process. The model was calibrated and optimized using previously published experimental data and kinetic parameter values obtained with a mixed, mesophilic (35 degrees C) methanogenic culture. Model simulations were used to predict the effect of nitrate reduction on the methanogenic fermentation process in batch, semi-continuous, and continuous flow reactors experiencing operational changes and/or system disturbances. The extended model clearly revealed the importance of substrate competition between denitrifiers and non-denitrifiers as well as the impact of N-oxide inhibition on process interactions between fermentation, methanogenesis, and denitrification.
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