Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The ligand-independent translocation assay: an enabling technology for screening orphan G protein-coupled receptors by arrestin recruitment.

Methods in enzymology·2006
Same author

High-content screening of known G protein-coupled receptors by arrestin translocation.

Methods in enzymology·2006
Same author

Compound classification using image-based cellular phenotypes.

Methods in enzymology·2006
Same author

A large T cell invagination with CD2 enrichment resets receptor engagement in the immunological synapse.

Journal of immunology (Baltimore, Md. : 1950)·2006
Same author

An unbiased cell morphology-based screen for new, biologically active small molecules.

PLoS biology·2005
Same author

Interface accumulation of receptor/ligand couples in lymphocyte activation: methods, mechanisms, and significance.

Immunological reviews·2002

Related Experiment Video

Updated: Jun 22, 2026

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
11:24

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples

Published on: October 31, 2025

Design and implementation of high-content imaging platforms: lessons learned from end user-developer collaboration.

Cynthia L Adams1, Michael D Sjaastad

  • 1Cytokinetics, Inc., South San Francisco, CA, USA. cindy.hazuka@sbcglobal.net

Combinatorial Chemistry & High Throughput Screening
|June 18, 2009
PubMed
Summary

Developing automated high-content screening and analysis (HCS/HCA) platforms requires careful planning and validation. Collaboration between technology providers and researchers accelerates the creation of effective HCS/HCA solutions for drug discovery.

More Related Videos

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology
09:55

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology

Published on: September 28, 2022

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

Related Experiment Videos

Last Updated: Jun 22, 2026

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
11:24

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples

Published on: October 31, 2025

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology
09:55

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology

Published on: September 28, 2022

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

Area of Science:

  • Biotechnology
  • Drug Discovery
  • Bioinformatics

Background:

  • Automated high-content screening and analysis (HCS/HCA) technologies are crucial for accelerating drug discovery.
  • The complexity of HCS/HCA platforms necessitates proactive design, building, and validation.
  • Effective collaboration between technology providers and end-users is essential for developing new HCS/HCA platforms.

Purpose of the Study:

  • To review the development history and considerations for HCS/HCA platforms.
  • To examine the evolution of specific HCS/HCA systems, including the Cytometrix Profiling System and Molecular Devices Corporation's "Complete Imaging Solution."
  • To highlight the benefits of collaborative development in creating customized HCS/HCA technologies.

Main Methods:

  • Historical review of HCS/HCA platform development.
  • Analysis of collaboration models between technology developers and research end-users.
  • Case study examination of Cytokinetics' and Molecular Devices Corporation's HCS/HCA system development cycles.

Main Results:

  • Successful HCS/HCA platform development relies on iterative design and validation processes.
  • Collaboration leads to cost-effective creation of tailored HCS/HCA technologies meeting specific industrial needs.
  • The review traces the development from initial concepts through multiple cycles to market consolidation.

Conclusions:

  • Proactive development and validation of HCS/HCA platforms are critical for efficient drug discovery.
  • Managed collaboration accelerates the creation of advanced HCS/HCA hardware and software.
  • This approach ensures the development of technologies that meet precise industrial requirements before commercialization.