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Related Experiment Video

Updated: Jun 22, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

SPR imaging for high throughput, label-free interaction analysis.

Christopher Lausted1, Zhiyuan Hu, Leroy Hood

  • 1Institute for Systems Biology, Seattle, Washington 98103, USA. clausted@systemsbiology.org

Combinatorial Chemistry & High Throughput Screening
|June 18, 2009
PubMed
Summary
This summary is machine-generated.

Surface plasmon resonance (SPR) imaging sensors offer label-free analysis of biomolecular interactions. This high-throughput method enables real-time monitoring of binding kinetics for DNA, RNA, proteins, and antibodies on microarrays.

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Last Updated: Jun 22, 2026

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biophysics

Background:

  • Surface plasmon resonance (SPR) has been a valuable tool for studying biomolecular binding and kinetics for two decades.
  • Label-free detection methods are crucial for accurate analysis of molecular interactions.

Purpose of the Study:

  • To highlight the adaptation of SPR sensors for high-throughput microarray analysis.
  • To showcase the utility of SPR imaging for real-time monitoring of biomolecular binding.

Main Methods:

  • Utilizing SPR-active microarrays for simultaneous analysis of multiple interactions.
  • Employing SPR imaging to visualize and quantify binding events in real-time.
  • Arraying diverse biomolecules including DNA, RNA, antibodies, and proteins as probes or targets.

Main Results:

  • Demonstrated the effectiveness of SPR imaging for label-free, real-time monitoring of biomolecular binding on microarrays.
  • Quantitatively analyzed binding kinetics for a wide range of biomolecules.
  • Established SPR imaging as a robust high-throughput screening tool.

Conclusions:

  • SPR imaging sensors provide a powerful, label-free platform for high-throughput analysis of biomolecular interactions.
  • The technology facilitates real-time kinetic studies of diverse molecular targets on microarrays.
  • This approach significantly advances the study of molecular binding in various biological applications.