Related Experiment Video
Updated: Jun 22, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Multi-contrast light profile microscopy for the depth-resolved imaging of the properties of multi-ply thin films
1Department of Chemistry, McGill University, 801 Sherbrooke St. W. Montreal, QC H3A 2K6, Canada. joan.power@mcgill.ca
Abstract:
Light profile microscopy (LPM) is a direct method for the spectral depth imaging of thin film cross-sections on the micrometer scale. LPM uses a perpendicular viewing configuration that directly images a source beam propagated through a thin film. Images are formed in dark field contrast, which is highly sensitive to subtle interfacial structures that are invisible to reference methods. The independent focusing of illumination and imaging systems allows multiple registered optical sources to be hosted on a single platform. These features make LPM a powerful multi-contrast (MC) imaging technique, demonstrated in this work with six modes of imaging in a single instrument, based on (1) broad-band elastic scatter; (2) laser excited wideband luminescence; (3) coherent elastic scatter; (4) Raman scatter (three channels with RGB illumination); (5) wavelength resolved luminescence; and (6) spectral broadband scatter, resolved in immediate succession. MC-LPM integrates Raman images with a wider optical and morphological picture of the sample than prior art microprobes. Currently, MC-LPM resolves images at an effective spectral resolution better than 9 cm(-1), at a spatial resolution approaching 1 microm, with optics that operate in air at half the maximum numerical aperture of the prior art microprobes.
More Related Videos
08:32Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks
Published on: January 26, 2024
10:42In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography (CT) and Light Microscopy (LM) Correlated with Scanning Electron Microscopy (SEM)
Published on: June 16, 2016
Related Concept Videos
Confocal Fluorescence Microscopy
Three-Dimensional Microscopy in Microbiology
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Super-resolution Fluorescence Microscopy
Total Internal Reflection Fluorescence Microscopy