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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

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Polarization-sensitive spectral-domain optical coherence tomography using a single line scan camera.

Barry Cense, Mircea Mujat, Teresa C Chen

|June 18, 2009

Summary

A new spectral-domain polarization-sensitive optical coherence tomography system significantly speeds up retinal nerve fiber layer birefringence measurements. This advanced imaging technology reveals location-dependent birefringence variations in the human retina, crucial for understanding optic nerve health.

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Published on: October 2, 2021

Area of Science:

Ophthalmology
Biomedical Optics
Medical Imaging

Background:

Polarization-sensitive optical coherence tomography (PS-OCT) measures tissue birefringence.
Previous time-domain (TD) PS-OCT showed human retinal nerve fiber layer (RNFL) birefringence varies spatially around the optic nerve head.
Understanding RNFL birefringence is important for diagnosing optic nerve diseases.

Purpose of the Study:

To develop and validate a faster spectral-domain (SD) PS-OCT system for measuring RNFL birefringence.
To assess the feasibility of SD-PS-OCT for mapping RNFL birefringence variations.
To compare the performance of the new SD-PS-OCT system with existing TD-PS-OCT.

Main Methods:

A novel spectral-domain polarization-sensitive optical coherence tomography system was designed using a single line scan camera and a Wollaston prism.
The system's speed was evaluated against a time-domain system.
Concentric circular scans were performed around the optic nerve head of a healthy volunteer to acquire RNFL data.

Main Results:

The developed SD-PS-OCT system is 60 times faster than TD-PS-OCT, reducing scan time from 72s to 1.2s for 12 circular scans.
Acquired data showed spatial variations in retinal thickness and double-pass phase retardation (DPPR) per unit depth.
RNFL DPPR per unit depth ranged from 0.18 to 0.40 degrees/µm, corresponding to birefringence values of 2.2 x 10⁻⁴ to 4.8 x 10⁻⁴ at 840 nm.

Conclusions:

The spectral-domain polarization-sensitive optical coherence tomography system offers a significantly faster and simplified approach for birefringence measurement.
The system successfully demonstrated location-dependent variations in RNFL birefringence, consistent with previous findings.
This advanced imaging technique holds promise for improved clinical assessment of optic nerve health.