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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jun 22, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Single-organelle tracking by two-photon conversion.

Wataru Watanabe, Tomoko Shimada, Sachihiro Matsunaga

|June 18, 2009

Summary

This summary is machine-generated.

Researchers developed a new method to track single mitochondria in living plant cells using advanced laser technology. This technique reveals how mitochondria move along cellular structures, offering insights into organelle dynamics.

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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

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Published on: June 30, 2018

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Last Updated: Jun 22, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Using Ex Vivo Live Imaging to Investigate Cell Divisions and Movements During Mouse Dental Renewal

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Published on: October 27, 2023

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Understanding intracellular object dynamics is crucial for cell biology.
Photoactivatable fluorescent proteins enable discrimination of specific intracellular objects via light-induced spectral changes.

Purpose of the Study:

To develop a technique for selective labeling and tracking of single organelles in living cells.
To investigate the mechanisms of mitochondrial movement in plant cells.

Main Methods:

Utilized two-photon conversion of a photoconvertible fluorescent protein (Kaede) with near-infrared femtosecond laser pulses.
Applied selective labeling to a single mitochondrion in a living tobacco BY-2 cell.
Tracked the dynamics of the labeled mitochondrion.

Main Results:

Demonstrated directed movement of individual mitochondria along cytoskeletons in plant cells.
Showed that actin filaments mediate mitochondrial movement.
Found that microtubules are not required for mitochondrial movement.

Conclusions:

The developed single-organelle labeling technique allows for precise tracking of organelle dynamics.
Revealed the cytoskeletal basis for mitochondrial motility in plants.
The technique has potential for tracking various cellular and intracellular structures.