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Updated: Jun 22, 2026

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity.

Jean-Christophe Baret¹, Oliver J Miller, Valerie Taly

¹Institut de Science et d'Ingénierie Supramoléculaires (ISIS), Université de Strasbourg, CNRS UMR 7006, 8 allée Gaspard Monge, BP 70028, F-67083, Strasbourg Cedex, France.

|June 18, 2009

Summary

We developed a microfluidic fluorescence-activated droplet sorter (FADS) for high-throughput cell sorting. This innovative system efficiently sorts single cells in droplets, achieving high purity and recovery rates for biotechnological applications.

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Last Updated: Jun 22, 2026

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

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Published on: November 4, 2016

Area of Science:

Biotechnology
Microfluidics
Biophysics

Background:

Traditional cell sorting methods face limitations in throughput and sample handling.
Microfluidic devices offer miniaturization and precise control for biological assays.
Fluorescence-activated sorting enables high-sensitivity detection and selection of specific cells.

Purpose of the Study:

To develop and validate a novel microfluidic fluorescence-activated droplet sorter (FADS).
To combine the advantages of microtitre-plate screening and fluorescence-activated cell sorting (FACS).
To achieve high-throughput, accurate sorting of single cells encapsulated in emulsion droplets.

Main Methods:

Single cells were compartmentalized within emulsion droplets.
Dielectrophoresis was used for fluorescence-activated sorting of droplets.
Sorting rates of up to 2000 droplets per second were achieved.
E. coli cells expressing reporter enzymes were used for system validation.

Main Results:

The FADS system demonstrated efficient sorting of E. coli cells at approximately 300 droplets s(-1).
The false positive error rate was less than 1 in 10(4) droplets.
Co-encapsulation of cells, not sorting errors, limited enrichment.
High sorting efficiency and cell recovery were achieved at low cell densities.

Conclusions:

The microfluidic FADS is a highly efficient platform for single-cell sorting.
The system offers a powerful tool for applications requiring high-throughput screening and cell selection.
The theoretical model accurately predicted sorting performance based on cell density and activity ratios.