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Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers

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A novel multishear microdevice for studying cell mechanics.

Lien Chau¹, Michael Doran, Justin Cooper-White

¹Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, 4072, Australia.

|June 18, 2009

Summary

This study introduces a new device for testing cell mechanics under various shear stresses. It reveals that higher shear stress increases von Willebrand factor secretion and reduces cell size in endothelial cells.

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A Microfluidic Technique to Probe Cell Deformability

A Microfluidic Technique to Probe Cell Deformability

Published on: September 3, 2014

Area of Science:

Biomedical Engineering
Cell Biology
Mechanobiology

Background:

Shear stress significantly impacts cell behavior, including morphology and function, affecting cell types like endothelial, smooth muscle, and osteoblast cells.
Existing shear devices typically evaluate only one shear stress level per experiment, limiting comprehensive analysis.

Purpose of the Study:

To introduce a novel shear device capable of simultaneously evaluating multiple shear stress levels.
To investigate the effects of a wide range of physiologically relevant shear stresses on human umbilical vein endothelial cells (HUVECs).

Main Methods:

Development of a new shear device allowing simultaneous evaluation of 10 different shear stresses (0.07-13 Pa).
Exposure of HUVECs to various shear stress profiles for 20 hours.
Analysis of von Willebrand factor (vWF) secretion and cellular morphology (cell and nuclear size, perimeter).

Main Results:

Increasing shear stress led to elevated vWF secretion in HUVECs, consistent with prior research.
HUVECs exposed to shear stresses above 0.5 Pa exhibited significantly higher vWF secretion and were at least 30% smaller in cell size compared to static controls.
Shear stresses below 0.3 Pa showed minimal changes in vWF and cell size compared to static conditions, while 0.07 Pa resulted in smaller cell size and lower vWF levels.

Conclusions:

The novel shear device enables rapid screening of cellular responses to a broad spectrum of shear stresses.
The device facilitates simultaneous evaluation of multiple shear stresses, offering a significant advantage over existing technologies for cell mechanics studies.