Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Randomized, Double-Blind, Placebo-Controlled, Global Phase III Trial of Talimogene Laherparepvec Combined With Pembrolizumab for Advanced Melanoma.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2022
Same author

Clinical implications of clonal chromosomal abnormalities in Philadelphia negative cells in CML patients after treated with tyrosine kinase inhibitors.

Cancer genetics·2019
Same author

A phosphoarray platform is capable of personalizing kinase inhibitor therapy in head and neck cancers.

International journal of cancer·2017
Same author

A Novel EGFR Extracellular Domain Mutant, EGFRΔ768, Possesses Distinct Biological and Biochemical Properties in Neuroblastoma.

Molecular cancer research : MCR·2016
Same author

Evaluation of a cost effective broth and selective agar combination for the detection of MRSA and Staphylococcus aureus from surveillance specimens using regular workflow.

Clinical laboratory science : journal of the American Society for Medical Technology·2014
Same author

A novel activating, germline JAK2 mutation, JAK2R564Q, causes familial essential thrombocytosis.

Blood·2014

Related Experiment Video

Updated: Jun 22, 2026

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
09:12

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

Comparing ImmunoCard with two EIA assays for Clostridium difficile toxins.

Edward L Chan1, Diane Seales, Hong Drum

  • 1LSU Health Sciences Center, 1501 Kings Highway, Shreveport, Louisiana 71130-3932, USA. echan@lsuhsc.edu

Clinical Laboratory Science : Journal of the American Society for Medical Technology
|June 19, 2009
PubMed
Summary

The ImmunoCard enzyme immunoassay (EIA) kit demonstrated superior sensitivity (100%) for detecting Clostridium difficile toxins A and B. This makes it a highly accurate and potentially cost-effective option for diagnosing C. difficile infections.

More Related Videos

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
10:12

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Published on: August 8, 2013

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
12:11

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Related Experiment Videos

Last Updated: Jun 22, 2026

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
09:12

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
10:12

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Published on: August 8, 2013

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
12:11

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Area of Science:

  • Clinical Microbiology
  • Diagnostic Assays
  • Infectious Diseases

Background:

  • Clostridium difficile is a significant cause of infectious diarrhea.
  • Accurate and rapid detection of C. difficile toxins is crucial for patient management.
  • Enzyme immunoassay (EIA) kits are commonly used for toxin detection.

Purpose of the Study:

  • To compare the performance of three commercially available EIA kits for detecting Clostridium difficile toxins in clinical stool specimens.
  • To evaluate sensitivity, specificity, and predictive values of each assay.

Main Methods:

  • A total of 287 stool specimens were analyzed using three EIA kits: Meridian EIA, ImmunoCard, and BioStar OIA.
  • Discrepant results were confirmed using a reference laboratory's tissue cytotoxin assay.

Main Results:

  • ImmunoCard exhibited 100% sensitivity and 98.2% specificity for detecting C. difficile toxins A and B.
  • Meridian EIA showed 99.1% sensitivity and 97.7% specificity.
  • BioStar OIA assay had 94% sensitivity and 98.8% specificity, missing seven positive samples.

Conclusions:

  • ImmunoCard offers the highest sensitivity and predictive values among the tested kits.
  • The kit provides faster results and greater convenience, potentially offering cost-effectiveness in various laboratory settings.