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Updated: Jun 22, 2026
07:52
Conjunctival Commensal Isolation and Identification in Mice
Published on: May 1, 2021
The Mycoplasma conjunctivae genome sequencing, annotation and analysis
Sandra P Calderon-Copete1, George Wigger, Christof Wunderlin
1Swiss Institute of Bioinformatics, Génopode-UNIL, 1015 Lausanne, Switzerland. Sandra.Calderon@unil.ch
BMC Bioinformatics
|June 19, 2009
Summary
Mycoplasma conjunctivae causes infectious keratoconjunctivitis (IKC) in sheep and wild animals. A new automated genome annotation pipeline aids in identifying potential therapeutic targets for this significant pathogen.
Area of Science:
- Microbiology
- Genomics
- Veterinary Medicine
Background:
- Mycoplasma conjunctivae is the cause of infectious keratoconjunctivitis (IKC).
- IKC is generally mild in domestic sheep but severe, potentially fatal, in wild Alpine caprinae.
- This pathogen poses a significant threat to protected wild species.
Purpose of the Study:
- To sequence and annotate the Mycoplasma conjunctivae genome.
- To develop and validate an automated genome annotation pipeline.
- To facilitate comparative genomics and identify potential therapeutic targets.
Main Methods:
- Genome sequencing using GS-FLX (454) and Sanger techniques.
- Development of an automated annotation pipeline using PERL scripts and various tools.
- Storage of annotations in a MySQL database.
Main Results:
- The annotated Mycoplasma conjunctivae genome sequence is available in the EMBL database (FM864216).
- The data is accessible via the mollicutes database MolliGen for comparative genomics.
- The automated pipeline successfully annotated the complete mycoplasma genome.
Conclusions:
- The developed automated pipeline is effective for annotating mycoplasma genomes.
- The annotated genome facilitates comparative analyses.
- The study identified potential biological targets, such as pathogenic proteins, for further investigation.
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