The Mycoplasma conjunctivae genome sequencing, annotation and analysis

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Conjunctival Commensal Isolation and Identification in Mice
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Conjunctival Commensal Isolation and Identification in Mice

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Sandra P Calderon-Copete1, George Wigger, Christof Wunderlin

  • 1Swiss Institute of Bioinformatics, Génopode-UNIL, 1015 Lausanne, Switzerland. Sandra.Calderon@unil.ch

BMC Bioinformatics
|June 19, 2009
PubMed
Abstract

Insights

Mycoplasma conjunctivae causes infectious keratoconjunctivitis (IKC) in sheep and wild animals. A new automated genome annotation pipeline aids in identifying potential therapeutic targets for this significant pathogen.

Area of Science:

  • Microbiology
  • Genomics
  • Veterinary Medicine

Background:

  • Mycoplasma conjunctivae is the cause of infectious keratoconjunctivitis (IKC).
  • IKC is generally mild in domestic sheep but severe, potentially fatal, in wild Alpine caprinae.
  • This pathogen poses a significant threat to protected wild species.

Purpose of the Study:

  • To sequence and annotate the Mycoplasma conjunctivae genome.
  • To develop and validate an automated genome annotation pipeline.
  • To facilitate comparative genomics and identify potential therapeutic targets.

Main Methods:

  • Genome sequencing using GS-FLX (454) and Sanger techniques.
  • Development of an automated annotation pipeline using PERL scripts and various tools.
  • Storage of annotations in a MySQL database.

Main Results:

  • The annotated Mycoplasma conjunctivae genome sequence is available in the EMBL database (FM864216).
  • The data is accessible via the mollicutes database MolliGen for comparative genomics.
  • The automated pipeline successfully annotated the complete mycoplasma genome.

Conclusions:

  • The developed automated pipeline is effective for annotating mycoplasma genomes.
  • The annotated genome facilitates comparative analyses.
  • The study identified potential biological targets, such as pathogenic proteins, for further investigation.

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