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Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
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Rapid Identification of Pathogens

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Related Experiment Video

Updated: Jun 22, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

[Multilocus sequence typing of methicillin-resistant Staphylococcus aureus].

Xiao-mei Yan1, Yi-xin Gu, Li-hua He

  • 1National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine]
|June 19, 2009
PubMed
Summary
This summary is machine-generated.

Multilocus sequence typing (MLST) reveals ST239 and ST5 as the most common methicillin-resistant Staphylococcus aureus (MRSA) clones. This method effectively differentiates MRSA from MSSA strains and identifies novel clones.

Related Experiment Videos

Last Updated: Jun 22, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Context:

  • Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant public health threat.
  • Understanding the genetic diversity of MRSA is crucial for effective control strategies.
  • Multilocus sequence typing (MLST) is a powerful tool for bacterial strain characterization.

Purpose:

  • To characterize methicillin-resistant Staphylococcus aureus (MRSA) strains using MLST.
  • To analyze the prevalence of specific sequence types (STs) in MRSA isolates from 2000 and 2005.
  • To differentiate MRSA from methicillin-sensitive Staphylococcus aureus (MSSA) based on allelic profiles.

Summary:

  • MLST analysis of 29 MRSA and 2 MSSA strains identified ST239 as the predominant MRSA clone in both 2000 and 2005.
  • ST5 emerged as a significant MRSA clone in 2005, representing 41.18% of new types.
  • MSSA strains exhibited distinct allelic profiles (ST6, ST630), differentiating them from MRSA.

Impact:

  • MLST provides an unambiguous method for assigning MRSA and MSSA isolates to known or novel clones.
  • The findings highlight the dynamic nature of MRSA clonal populations.
  • Further studies with larger strain collections are warranted to enhance our understanding of MRSA epidemiology.