[Preparation of gene chip for detecting different expression genes involved in aflatoxin biosynthesis]
1College of Life Science, Jiangxi Normal University of Science and Technology, Nanchang 330013, China.
Objective:
To develop the methodology of gene chip to analyse genes involved in aflatoxin biosynthesis.
Methods:
In comparing reversed transcriptional PCR with gene chip, the gene chip was used to detect genes involved in aflatoxin biosynthesis.
Results:
After arrayed the slide was incubated in water for 2 hours, exposed to a 650 mJ/cm2 of ultraviolet irradiation in the strata-linker for 30 s, roasted under 80 degrees C for 2 hours in oven, pre-hybridized for 45 minutes and dealt with other procedures. Finally, the slide was hybridized with fluor-derivatized sample at 42 degrees C for 16 hours.
Conclusion:
With the reasonable probe design and applicable protocol, the gene chip was prepared effectively for research on genes involved in aflatoxin biosynthesis.
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