Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oogenesis02:07

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The expression of lysophosphatidic acid and C-reactive protein in the course of the vulvar squamous cell carcinoma and the vulvar lichen sclerosus.

Ginekologia polska·2026
Same author

Risk Factor Levels and the Burden of Skin Melanoma in Poland with Predictions Regarding the 2020-2030 Perspective.

Journal of clinical medicine·2026
Same author

Regional Trends and Forecasts of Pancreatic Cancer Incidence in Poland: A Voivodeship-Level Analysis of Risk Factors.

Journal of clinical medicine·2026
Same author

Chronic inflammatory activity in women with normogonadotropic anovulation complicated by subclinical thyroid dysfunction: a prospective cohort study.

Frontiers in endocrinology·2026
Same author

Levels of Systemic Inflammatory Markers in Women with Cervical Cancer Undergoing Definitive Chemoradiotherapy Depend Primarily on Pre-treatment and Post-teletherapy Tumor Volume, Rather than on the Prescribed Radiation Dose.

Current pharmaceutical biotechnology·2026
Same author

Hyperandrogenaemia and Systemic Low-Grade Inflammation in Normogonadotropic Anovulation: A Prospective Cohort Study.

Archives of medical research·2025

Related Experiment Video

Updated: Jun 22, 2026

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization
11:08

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization

Published on: April 7, 2023

DFF45 expression in ovarian endometriomas.

Tomasz Banas1, Krzysztof Skotniczny, Antoni Basta

  • 1Jagiellonian University, Chair of Obstetrics and Gynecology, 23 Kopernika Street, 30-501 Krakow, Poland. tbanas@mp.pl

European Journal of Obstetrics, Gynecology, and Reproductive Biology
|June 19, 2009
PubMed
Summary

Ovarian endometriosis shows decreased levels of DNA fragmentation factor 45 (DFF45), a protein crucial for apoptosis. This reduction may contribute to apoptosis resistance and disease progression in endometriosis patients.

More Related Videos

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time
14:25

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time

Published on: May 20, 2014

Related Experiment Videos

Last Updated: Jun 22, 2026

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization
11:08

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization

Published on: April 7, 2023

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time
14:25

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time

Published on: May 20, 2014

Area of Science:

  • Gynecology
  • Cell Biology
  • Molecular Biology

Background:

  • Endometriosis affects up to 30% of women of reproductive age, commonly localized in the ovaries.
  • Ectopic endometrial cells in endometriosis exhibit abnormal proliferation and impaired apoptosis.
  • The DNA fragmentation factor 45 (DFF45) plays a critical role in apoptosis regulation and DFF40 synthesis.

Purpose of the Study:

  • To investigate the expression levels of DFF45 in human ovarian endometriosis.
  • To compare DFF45 levels between normal endometrium and endometriotic tissues.

Main Methods:

  • Western blot and immunohistochemistry were employed to analyze DFF45 expression.
  • Tissue samples were obtained from 43 women with endometriosis and 81 controls.

Main Results:

  • DFF45 expression was confirmed in both normal endometrium and ovarian endometriosis.
  • Ovarian endometriosis exhibited significantly lower DFF45 levels compared to normal eutopic endometrium.
  • Unlike normal endometrium, ovarian endometriosis showed no difference in DFF45 staining between glandular and stromal layers.

Conclusions:

  • Decreased DFF45 levels in ovarian endometriosis may indicate an apoptosis-resistant mechanism.
  • This reduction in DFF45 could be a contributing factor to the progression of endometriosis.