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A Protocol for Analyzing Hepatitis C Virus Replication
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A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Detection and quantification of hepatitis C virus (HCV) by MultiCode-RTx real-time PCR targeting the HCV 3'

Elizabeth K Mulligan1, J J Germer, Max Q Arens

  • 1EraGen Biosciences, Inc., Madison, WI 53717, USA.

Journal of Clinical Microbiology
|June 19, 2009
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...