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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
X-ray Crystallography02:18

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The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
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Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

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Related Experiment Video

Updated: Jun 22, 2026

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
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Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Advanced phase-contrast imaging using a grating interferometer.

Samuel Alan McDonald1, Federica Marone, Christoph Hintermüller

  • 1Swiss Light Source, Paul Scherrer Institut, 5232 Villigen PSI, Switzerland.

Journal of Synchrotron Radiation
|June 19, 2009
PubMed
Summary
This summary is machine-generated.

Phase-sensitive X-ray imaging, specifically differential phase-contrast (DPC) tomography, enhances contrast for detailed sample analysis. This advanced technique at the Swiss Light Source enables faster, high-throughput imaging with reduced radiation dose.

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Last Updated: Jun 22, 2026

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Area of Science:

  • Medical Imaging
  • X-ray Tomography
  • Materials Science

Background:

  • Conventional X-ray imaging relies on absorption, limiting contrast for certain samples.
  • Phase-sensitive X-ray methods offer superior contrast, revealing otherwise hidden details.
  • Differential phase-contrast (DPC) imaging utilizes grating interferometry for enhanced sensitivity.

Purpose of the Study:

  • To enhance the capabilities of the TOMCAT beamline for high-throughput, phase-sensitive X-ray imaging.
  • To develop fast acquisition and post-processing methods for DPC tomography.
  • To explore novel applications of DPC, including aqueous environments and widefield imaging.

Main Methods:

  • Integration of DPC imaging with a grating interferometer at the TOMCAT beamline.
  • Implementation of fast data acquisition (9000 projections in 20 min) and post-processing pipeline.
  • Development of an aquarium setup for in-situ measurements in aqueous environments.
  • Demonstration of local DPC tomography and widefield DPC imaging.
  • Extraction of darkfield images from DPC scans.

Main Results:

  • Achieved high-throughput DPC tomography with reduced radiation dose.
  • Enabled imaging in aqueous environments using a novel aquarium design.
  • Demonstrated artifact-free local DPC tomography for high-magnification imaging.
  • Successfully implemented widefield DPC imaging, doubling the field of view.
  • Showcased visualization of soft tissue features, including the rat substantia nigra.
  • Extracted valuable darkfield contrast information from DPC scans.

Conclusions:

  • The enhanced DPC imaging capabilities at TOMCAT significantly advance X-ray tomography for various applications.
  • Fast acquisition and novel sample environments broaden the scope of phase-sensitive imaging.
  • Widefield and local tomography techniques offer unprecedented versatility in sample analysis.
  • The integration of darkfield imaging provides complementary information, enhancing diagnostic potential.