A flow-injection chemiluminescent method for the evaluation of the antioxidant activity of 5'-nucleotides

In the present work, a simple chemiluminescence (CL) method coupled with flow-injection analysis for the evaluation of antioxidant activity of 5'-nucleotides (5'-AMP, 5'-CMP, 5'-GMP, 5'-UMP) was proposed. It is based on inhibition effect of the studied substances on CL emission of luminol-potassium ferricyanide-pyrogallol. Experiments were performed to evaluate the nature of the inhibition by 5'-nucleotides of the CL reaction and their antioxidant activities. Based on the experimental results, it was observed that 5'-nucleotides are available antioxidants that could effectively scavenge superoxide anion free radicals in a concentration-dependent way. This will provide a basis for further development of the use of nucleotides.