Identification of a novel growth hormone binding protein mRNA in rat liver

The presence of highly specific serum binding proteins for growth hormone (GH) has been well characterized in many species. In the rat the major growth hormone-binding protein (GH-BP) is a truncated, variant form of the target tissue GH receptor and is derived by an alternative mRNA splicing event. The GHBP mRNA is coexpressed in all tissues expressing the full length GH receptor. In the present study, we have made an oligonucleotide probe to the unique hydrophilic tail of the rat GHBP mRNA (1.2kb) and identified a novel GHBP-like mRNA of 2.6 kb transcript in addition to the 1.2 kb transcript. This unique 2.6 kb transcript was expressed/detected only in rat liver. There was no significant difference in abundance between the sexes or during pregnancy, implying that this transcript may be regulated independently of the 1.2 kb mRNA. The 2.6 kb transcript was clearly identifiable in the fetus, as was the 1.2 kb transcript, but showed virtually no change in abundance with age, in sharp contrast to the 1.2 kb mRNA, which has a distinct developmental pattern, being low in the fetus and peaking early postnatally. RNAse H treatment suggested that this 2.6 kb transcript is polyadenylated. A corresponding 2.6 kb mRNA has been detected using a longer cDNA or cRNA probe for the GH-binding domain of the rat GHR/GHBP. These data collectively suggest that the 2.6 kb mRNA transcript is a bonafide but tissue-specific GHBP mRNA and that the 1.2 and 2.6 kb mRNAs are likely to differ primarily with respect to the length of the 3' untranslated region of the sequence.