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Updated: Jun 22, 2026

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes
Published on: September 15, 2018
Peptide-mediated targeting of hepatocytes via low density lipoprotein receptor-related protein (LRP)
James J Ludtke1, Alex V Sokoloff, So Wong
1Waisman Center, Departments of Pediatrics and Medical Genetics, University of Wisconsin-Madison, Madison, WI 53705, USA.
Abstract:
It has previously been reported that a peptide sequence of T7 phage protein p17 mediates uptake of its cargo by liver parenchymal cells. The aim of this study was to identify the phage-binding receptor. The involvement of LRP was confirmed by the observations that phage binding to Hepa 1c1c7 cells was inhibited by the LRP-binding receptor-associated protein, LRP-deficient mouse embryonic fibroblasts bound phage with lower efficiency than their wild-type counterparts, and using mouse models with ablated LRP liver expression. The identification of LRP as a cognate receptor for this sequence offers a new ligand-receptor combination for hepatocyte delivery of therapeutic agents.
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