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Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
Published on: October 9, 2016
Anionic polymers of the cell wall of Bacillus subtilis subsp. subtilis VKM B-501(T)
A S Shashkov1, N V Potekhina, S N Senchenkova
1Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia.
Abstract:
Teichoic acid and disaccharide-1-phosphate polymer were identified in the cell walls of Bacillus subtilis subsp. subtilis VKM B-501(T). The teichoic acid represents 1,3-poly(glycerol phosphate) 80% substituted by alpha-D-glucopyranose residues at O-2 of glycerol. The linear repeating unit of disaccharide-1-phosphate polymer contains the residues of beta-D-glucopyranose, N-acetyl-alpha-D-galactosamine, and phosphate and has the following structure: -6)-beta-D-Glcp-(1-->3)-alpha-D-GalpNAc-(1-P-. The structures of two anionic polymers were determined by chemical and NMR-spectroscopic methods. The 1H- and 13C-NMR spectral data on disaccharide-1-phosphate polymer are presented for the first time.
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