Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tumour necrosis factor-α induces macromolecule translocation in HIV-derived duodenal organoids.

Frontiers in immunology·2025

Same author

Effect of claudin-1 or -3 expression on cation and water channel properties of claudin-2.

Biochimica et biophysica acta. Molecular cell research·2025

Same author

Matrix-free human 2D organoids recapitulate duodenal barrier and transport properties.

BMC biology·2025

Same author

Special Issue "The Tight Junction and Its Proteins: From Structure to Pathologies".

International journal of molecular sciences·2024

Same author

Oral curcumin ameliorates acute murine campylobacteriosis.

Frontiers in immunology·2024

Same author

Expression and Localization Profiles of Tight Junction Proteins in Immune Cells Depend on Their Activation Status.

International journal of molecular sciences·2024

Same journal

Multiomics Profiling During Autoimmune Demyelination Highlights a Complex Regulatory Role for Ataxin-1 in B Cells.

Annals of the New York Academy of Sciences·2026

Same journal

Global Trends in Light Pollution and Their Relationship With Socioeconomic Factors.

Annals of the New York Academy of Sciences·2026

Same journal

Wired for Corruption: Inter-Brain Synchrony Encodes Bribery-Related Value Information and Predicts Bribery Agreement.

Annals of the New York Academy of Sciences·2026

Same journal

LM-YOLO: A Lightweight Multi-Scale Enhanced Model for Forest Smoke Detection Using Unmanned Aerial Vehicles.

Annals of the New York Academy of Sciences·2026

Same journal

Polyrhythm Perception and Production: A Scoping Review.

Annals of the New York Academy of Sciences·2026

Same journal

DARTS-CNN-BiLSTM: Intelligent Fault Diagnosis for Computer Numerical Control Machine Tool Feed System.

Annals of the New York Academy of Sciences·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 1, 2026

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Published on: May 26, 2021

Tight junctions: molecular structure meets function.

Jörg-Dieter Schulzke¹, Michael Fromm

¹Department of General Medicine, Campus Benjamin Franklin, Freie Universität and Humboldt-Universität, Berlin, Germany.

Annals of the New York Academy of Sciences

|June 23, 2009

Summary

This summary is machine-generated.

Tight junctions form selective cell barriers regulating transport via proteins like claudins. Their composition and function are crucial for physiological adaptation and disease processes.

More Related Videos

$Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy$

Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy

Published on: September 11, 2014

Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

Published on: February 10, 2014

Related Experiment Videos

Last Updated: Jan 1, 2026

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Published on: May 26, 2021

$Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy$

Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy

Published on: September 11, 2014

Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

Published on: February 10, 2014

Area of Science:

Cell biology
Biophysics

Background:

Tight junctions (TJs) are crucial intercellular structures in epithelial and endothelial cells, forming selective paracellular barriers.
These barriers regulate the passage of solutes, immune cells, and therapeutic drugs.
TJs comprise proteins with transmembrane domains and extracellular loops that mediate cell adhesion and channel formation.

Discussion:

The molecular composition and ultrastructure of TJs are dynamically regulated by intracellular scaffolding proteins and the cytoskeleton.
This regulation is essential for normal physiological adaptation of epithelial and endothelial tissues.
Dysregulation of TJ structure and function is implicated in numerous disease pathologies.

Key Insights:

Key TJ proteins include occludin, tricellulin, and over 24 claudin family members.
These proteins feature four transmembrane domains and two extracellular loops, enabling zipper-like interactions between adjacent cells.
TJs exhibit dual roles: sealing the cell layer and forming selective paracellular channels.

Outlook:

Understanding TJ regulation offers therapeutic potential for diseases involving barrier dysfunction.
Further research into TJ protein interactions and regulatory mechanisms is warranted.
Targeting TJs could enhance drug delivery across epithelial and endothelial barriers.