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Related Experiment Video

Updated: Jun 22, 2026

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

Knocking in the NLRP3 inflammasome.

Stephen E Girardin¹

¹Department of Laboratory Medicine and Pathobiology, Medical Sciences Building, University of Toronto, Toronto, Ontario, Canada. stephen.girardin@utoronto.ca

|June 23, 2009

Summary

Researchers characterized Nlrp3 gene-targeted mice with mutations similar to those causing human autoinflammatory syndromes. This study provides a valuable model for investigating NLRP3-associated diseases.

Area of Science:

Immunology
Genetics
Molecular Biology

Background:

Mutations in the human NLRP3 gene are linked to autoinflammatory syndromes, specifically those involving interleukin-1beta.
Understanding the function of NLRP3 is crucial for developing treatments for these conditions.

Purpose of the Study:

To characterize Nlrp3 gene-targeted mice that carry mutations mirroring those found in human NLRP3-associated autoinflammatory diseases.
To establish a preclinical model for studying the pathogenesis and potential therapies of these syndromes.

Main Methods:

Generation of Nlrp3 gene-targeted mouse models.
Phenotypic analysis of the generated mouse models.
Assessment of inflammasome activation and cytokine production.

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Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

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Evaluation of Caspase Activation to Assess Innate Immune Cell Death

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Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

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Published on: May 21, 2018

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

Main Results:

The characterized Nlrp3 gene-targeted mice successfully mimic human disease-causing mutations.
These mouse models exhibit key features of autoinflammatory syndromes.
Analysis revealed altered inflammasome activity and cytokine profiles.

Conclusions:

Nlrp3 gene-targeted mice provide a robust preclinical model for studying human autoinflammatory diseases.
This research facilitates further investigation into NLRP3 inflammasome function and therapeutic strategies.