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Tracking transplanted cells in live animal using upconversion fluorescent nanoparticles.

Niagara Muhammad Idris1, Zhengquan Li, Lei Ye

  • 1Division of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore 117574, Singapore.

Biomaterials
|June 23, 2009
PubMed
Summary

New upconversion fluorescent nanoparticles enable dynamic tracking of live myoblast cells in vitro and in vivo. This breakthrough allows real-time observation of cell migration and fate for improved cell transplantation therapies.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Cell transplantation shows promise for treating diseases, necessitating methods to monitor cell behavior and effectiveness.
  • Tracking transplanted cells in real-time is crucial for assessing therapeutic outcomes and understanding cell dynamics.

Purpose of the Study:

  • To develop and evaluate upconversion fluorescent nanoparticles (silica/NaYF(4):Yb,Er) as a novel probe for dynamic live cell tracking.
  • To demonstrate the capability of these nanoparticles for in vitro and in vivo imaging of myoblast cells.

Main Methods:

  • Loading myoblast cells with silica/NaYF(4):Yb,Er nanoparticles.
  • Confocal imaging, spectrophotometry, and inductively coupled plasma analysis to confirm intracellular uptake.
  • Time-lapse confocal microscopy for in vitro dynamic imaging of cell migration.
  • In vivo confocal imaging in a mouse model to track transplanted cells in blood vessels and deep tissue.

Main Results:

  • Nanoparticles confirmed intracellular uptake and exhibited photobleaching resistance.
  • Successful real-time tracking of in vitro myoblast cell migration for 5 hours.
  • In vivo imaging visualized nanoparticle-loaded cells in blood vessels and identified them deep within muscle tissue (1300 microm).
  • Spatiotemporal migration of transplanted cells was captured for up to 7 days post-delivery in vivo.

Conclusions:

  • Silica/NaYF(4):Yb,Er nanoparticles serve as a robust fluorescent probe for live cell tracking.
  • This technology offers superior in vitro and in vivo dynamic imaging capabilities for cell transplantation research.
  • Enables unobtrusive, long-term monitoring of cell dynamics within native tissues.